Preservation of brewers yeast Part I: What role does intracellular trehalose content play?
The results presented here indicate that, of itself, intracellular trehalose content of yeast is not intimately related to the survival of cells in physiologically unfavourable situations. It can be shown, on the one hand, that trehalose acts as a reserve carbohydrate in starvation conditions. On the other hand, other parameters such as yeast strain, selection of preserving agent, composition of pre-propagation medium, the movement and aeration of cells during pre-propagation and pre-propagation age are found to be of considerable importance for successful preservation. Future investigations should thus be directed more specifically towards the composition of cell membranes.
The most commonly used yeast strain storage method is still to store them on slant agar.g.
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