Pre-enrichment for PCR detection of beer-spoiling yeast – possibilities and pitfalls
Rapid and reliable | Detecting yeasts that are detrimental to beverages is essential when ensuring the microbiological quality of the brewing process and finished products. In the current study, the growth characteristics of all important types of spoilage yeast were comprehensively analyzed under selective and non-selective enrichment conditions. The optimal duration of pre-enrichment for sensitive detection by real-time PCR as well as factors interfering with spoilage yeast growth could be determined.
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