Cold-tolerant yeast strains for cider and wine makers

The Finnish company developed new cold-tolerant hybrid strains that enable fermentation at lower and higher temperatures than before. In 2015 the company generated the first new lager brewing yeast strains in 500 years, and has now applied the knowledge obtained to create new yeast strains for the production of wine and cider. A key characteristic of these strains is that they can tolerate a wide range of temperatures from 10 to 37 °C. Importantly, the low temperature range reduces the risk of contamination during fermentation, possibly allowing for reduced sulphate use.

The tolerance to higher temperatures facilitates large-scale production in active dry yeast form. The wines and ciders produced with these strains are characterized by an increased aromatic complexity.

The ability of a yeast strain to ferment efficiently at low temperature is a desired feature in alcoholic fermentation. Cold fermentations have been used for centuries in the production of lager beer with the lager yeast Saccharomyces pastorianus. The ability of this species to ferment at low temperature is a result of it being a hybrid between an ale yeast and the cold-tolerant wild yeast Saccharomyces eubayanus.

Scientists at the research and technology company have now demonstrated that this combination of parents can also be effectively used for wine and cider fermentations. A wine yeast strain was crossed with the cold-tolerant parent of the lager yeast and the hybrids were tested for cider and wine fermentation.

The results showed that due to the wider range of temperatures tolerated by these species the aromatic properties of the cider and wine can be modulated by varying the fermentation temperature. White wine and cider, for example, benefit from low-temperature fermentations, both for reduced risk of contamination but also for an improved aromatic profile. Undesirable flavours that are typical of the wild parent are eliminated after hybridization and large-scale production is facilitated.

This natural, non-GM approach can be used for tailor-made generation of new strains by careful selection of the parent strains with desirable features. After being successfully applied to beer, wine and cider production, this technique is now being assessed for its use in the baking industry, where yeast must survive for extended periods in frozen dough.